Written in English
|Statement||by John Morris Johnson.|
|The Physical Object|
|Pagination||108 leaves, bound :|
|Number of Pages||108|
Graduation date: Contracting nucleolar vacuoles are a general and consistent\ud feature of somatic tobacco cells growing in microcultures. Nucleolar\ud vacuoles were studied in cells from callus tissues of two\ud single-cell clones, H and H, of hybrid tobacco . Johnson JM, Jones LE. Behavior of nucleoli and contracting nucleolar vacuoles in tobacco cells growing in microculture. Am J Bot. Feb; 54 (2)– LAFONTAINE JG. Structure and mode of formation of the nucleolus in meristematic cells of Vicia faba and Allium cepa. J Biophys Biochem Cytol. Nov 25; 4 (6)–Cited by: Nucleolar vacuoles in eight regions of the root apex of Zea mays have been studied. The volume of the vacuoles ranges from about 1 μm3 to μm3 with a mode value between and μm3. Single cells isolated from pith callus of fresh stem of hybrid tobacco (Nicotiana glutinosa ♂ x N. tabacum ♀) gave rise to small colonies of cells in microcultures which upon transfer to agar medium produced clones of callus tissue. These single-cell clones differentiated roots, and shoots with green leaves, on a completely defined nutrient medium.
Tobacco mosaic virus (TMV) and the mosaic disease induced by it have been studied ed cells and tissues growing in vitro have been increasingly used to study this virus and other viruses for their host cell interactions. Download: Download full-size image Fig. on micrograph of a very mild strain of TMV from tissue culture showing typical rods of TMV. × 40, We tested if different classes of vacuolar cargo reach the vacuole via distinct mechanisms by interference at multiple steps along the transport route. We show that nucleotide-free mutants of low molecular weight GTPases, including Rab11, the Rab5 members Rha1 and Ara6, and the tonoplast-resident Rab7, caused induced secretion of both lytic and storage vacuolar cargo. In situ analysis in . The first value was obtained on tobacco by Boiler and Kende . To overcome the problem of the very low quantities of membranes recovered in pure tonoplast fractions, tobacco cells were incu- bated with tritiated choline to label membrane phospholipids prior to protoplast and vacuole iso- lation [5, 18]. Multiple Vacuoles in Plant Cells. As discussed, seed plants have two principal, functionally distinct vacuole types, LVs and PSVs (Becker, ; Ibl and Stoger, ). This has raised questions about whether the two vacuoles co-exist in cells. To address this question, the two vacuole .
Plant cell vacuoles are widely diverse in form, size, content, and functional dynamics, and a single cell may contain more than one kind of vacuole. Although major morphological differences were recorded by the very first microscopists, it has been commonly assumed that all vacuoles have the same origin and belong to a common group. Paris and co-workers showed that, in root tips of barley and pea seedlings and in mature tobacco plants, two distinct vacuoles coexist in the same cell, one containing α-TIP on the tonoplast and lectin as cargo called the PSV and another defined by γ-TIP on the tonoplast and the protease aleurain as cargo called the lytic vacuole (Paris et al. Somatic cell nuclear transfer, technique in which the nucleus of a somatic (body) cell is transferred to the cytoplasm of an enucleated egg (an egg that has had its own nucleus removed). Once inside the egg, the somatic nucleus is reprogrammed by egg cytoplasmic factors to . Somatic cell nuclear transfer (SCNT) is a technique by which the nucleus of a differentiated cell is introduced into an oocyte from which its genetic material has been removed by a process called enucleation. In mammals, the reconstructed embryo is artificially induced to initiate embryonic development (activation).